Bisard is accused of being drunk when he crashed his squad car into three motorcyclists, killing Eric Wells in August 2010. He faces a reckless homicide charge.
Last month, officials discovered that second vial of blood had been moved from a refrigerator and placed on the shelf of an unrefrigerated annex room. That discovery led to Paul Ciesielski's resignation as police chief.
Bisard's attorney, John Kautman, said the second vial is unusable. He now wants the judge to block tests.
The first vial was ruled inadmissible because it had been drawn at the Methodist Occupational Health facility, a clinic which was not certified to perform such a procedure.
It's not clear when the judge will rule on the motion.